High-Speed Video-Microscopy Training
Welcome to the BEAT-PCD training modules on high-speed video-microscopy (HSVM). In Chapter 1, we introduce the basics of high-speed video-microscopy and why it matters. Chapter 2 covers why sample quality is key, and Chapter 3 shows how cell culture can help with diagnosis. Chapters 4 and 5 guide you through low-magnification checks and spotting normal ciliary edges and beat patterns. In Chapter 6, we look at cilia orientation and how it reveals dyskinesia. Finally, Chapter 7 helps you identify primary ciliary dyskinesia (PCD) using everything you’ve learned.
Training Material
The training is set up as a playlist on the BEAT-PCD Youtube channel [See below]. You can also jump to specific chapters using the links below, but we recommend following the order for the best learning experience.
Disclaimer: This training module is offered in English and is approximately 30 minutes long. Please note that sound and video quality may be slightly reduced on this platform compared with the original recordings and YouTube's compression may affect image sharpness. For optimal clarity, we recommend avoiding full-screen mode.
Intro - Welcome to BEAT-PCD HSVM training modules
Chapter 1 - Introduction to high-speed video microscopy
Chapter 2 - Sample quality is important
Chapter 3 - Cell culture is a diagnostic aid
Chapter 4 - Low magnification sample assessments
Chapter 5 - Normal edges and ciliary beat pattern
Chapter 6 - Orientation of cilia help to detect ciliary dyskinesia
Chapter 7 - Identifying PCD
End of training quiz
Great work completing the modules! Now put your skills to the test with our quick quiz – see how much you’ve mastered [Click here]
Disclaimer: This quiz is designed to evaluate engagement with and comprehension of the training material. It does not serve as an accredited examination and does not confer certification, professional recognition, or competency validation.
Supporting documents
Please find here the transcript for the videos [Click here]
We have also included a document with detailed descriptors to support your learning and help guide your assessments throughout the training [Click here]
Acknowledgement
BEAT-PCD would like to thank Professor Chris O'Callaghan (UCL) for conceiving the training programme, undertaking the initial video editing, and writing and narrating the transcript. Dr Robert Hirst (University of Leicester) processed ciliated samples, performed data analysis, and recorded the videos used throughout the training. The videos were generated within the NHS Centre for PCD Diagnosis and Research at the University of Leicester. The content chapters were edited and the quiz developed by Dr Claire Jackson and Prof Amelia Shoemark, with review and input from BEAT-PCD Work Package 3 members (Dr Panayiotis Kouis, Dr Nisreen Rumman and Dr Mathieu Bottier). Dr Mathieu Bottier also provided additional BEAT-PCD website design and video editing.
References
Stannard WA, Chilvers MA, Rutman AR, et al. Diagnostic testing of patients suspected of primary ciliary dyskinesia. Am J Respir Crit Care Med 2010; 181: 307–314.
Papon J-F, Bassinet L, Cariou-Patron G, et al. Quantitative analysis of ciliary beating in primary ciliary dyskinesia: a pilot study. Orphanet J Rare Dis 2012; 7: 78.
Pifferi M, Bush A, Montemurro F, et al. Rapid diagnosis of primary ciliary dyskinesia: cell culture and soft computing analysis. Eur Respir J 2013; 41: 960–965.
Rubbo B, Shoemark A, Jackson CL, et al. Accuracy of high-speed video analysis to diagnose primary ciliary dyskinesa. Chest 2019; 155: 1008–1017.
Coles JL, Thompson J, Horton KL, et al. A revised protocol for culture of airway epithelial cells as a diagnostic tool for primary ciliary dyskinesia. J Clin Med 2020; 9: 3753.
Baz-Redón N, Rovira-Amigo S, Fernández-Cancio M, et al. Immunofluorescence analysis as a diagnostic tool in a Spanish cohort of patients with suspected primary ciliary dyskinesia. J Clin Med 2020; 9: 3603.
Guo Z, Chen W, Wang L, et al. Clinical and genetic spectrum of children with primary ciliary dyskinesia in China. J Pediatr 2020; 225: 157–165.
Jackson CL, Bottier M. Methods for the assessment of human airway ciliary function. Eur Respir J. 2022 Jul 28;60(1):2102300
Join the HSVM Discussion on Slack
Additional questions about HSVM can be discussed through the “Work Package 3 – Improving PCD Diagnosis” Slack workspace. This collaborative platform allows BEAT-PCD members to ask questions, share challenging HSVM cases, and exchange protocols, images, videos, and resources. The workspace is moderated regularly by WP3 members, and participants are encouraged to focus on evidence-based discussion.
If you would like to join, please contact the BEAT-PCD Communications Officer (Dr Mathieu Bottier) to receive an invite.